2024 Cell signaling tech - Cell Signaling Technology (CST) is a different kind of life sciences company—one founded, owned, and run by active research scientists, with the highest standards of product and service quality ...

 
Lanes 1 & 2 are 5% input. Confocal immunofluorescent analysis of NCI-H1299 cells, untreated (left) or λ-phosphatase-treated (right), using Phospho-DRP1 (Ser616) Antibody (green). Actin filaments have been labeled with DY-554 phallodin (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).. Cell signaling tech

The c-Myc Antibody confirms silencing of c-Myc expression, and the p42 MAPK Antibody is used to control for protein loading and siRNA specificity. Western blot analysis of extracts from HeLa, BaF3 and NBT-11 cells, using c-Myc Antibody. Simple Western™ analysis of lysates (1 mg/mL) from HeLa cells using c-Myc Antibody #9402.If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.Monoclonal Antibody for studying ACTA2 (alpha actin, smooth muscle). Cited in 560 publications. Validated for WB, WB, IP, IHC, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, α-Smooth Muscle Actin (D4K9N) XP® Rabbit Monoclonal Antibody (CST #19245) is ready to ship.Species Cross-Reactivity Key: Polyclonal Antibody for studying eNOS. Cited in 167 publications. Validated for Western Blotting. Highly specific and rigorously validated in-house, eNOS Antibody (CST #9572) is ready to ship.A complete, step-by-step walkthrough of the CUT&RUN Protocol included with the CUT&RUN Assay kit (#86652) from Cell Signaling Technology (CST). • Visit the ...Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min. If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation. Checking the Cell Signaling Technology ® (CST ®) website, you are in luck! There are mouse reactive flow-validated antibody conjugates available against each target: CD4 …Immunoprecipitation of NF-kB p65 from CHO cell extracts. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is NF-κB p65 (D14E12) XP ® Rabbit mAb, #8242. Western blot was performed using NF-κB p65 (L8F6) Mouse mAb, #6956.Checking the Cell Signaling Technology ® (CST ®) website, you are in luck! There are mouse reactive flow-validated antibody conjugates available against each target: CD4 …Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses, including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182.Monoclonal Antibody for studying Flag-Tag. Cited in 478 publications. Validated for WB, IP, IHC, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, DYKDDDDK Tag (9A3) Mouse Monoclonal Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) (CST #8146) is ready to ship.Cell Signaling Technology (CST) is a different kind of life sciences company—one founded, owned, and run by active research scientists, with the highest standards …PAX2 is involved in the development of the nervous and excretory systems including the kidney and urogentical tract, the optic stalk, ear, midbrain-hindbrain junction, and the spinal cord (5,6). Lang, D. et al. (2007) Biochem Pharmacol 73, 1-14. Robson, E.J. et al. (2006) Nat Rev Cancer 6, 52-62. Background. Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation ... Wash sections in wash buffer for 5 min. Block each section with 100–400 µl of preferred blocking solution for 1 hr at room temperature. Remove blocking solution and add 100–400 µl primary antibody diluted in SignalStain ® Antibody Diluent ( #8112) to each section. Incubate overnight at 4°C.Mar 14, 2023 · A complete, step-by-step walkthrough of the CUT&RUN Protocol included with the CUT&RUN Assay kit (#86652) from Cell Signaling Technology (CST). • Visit the ... Aliquoting of 10x buffer is recommended if many small experiments are to be performed. 2. Thaw 10x buffer at 24-30°C, mixing end-over-end. 3. Dilute 10X Cell Lysis Buffer to a 1X solution using ddH2O. This product supplies enough 10X material to make 150mls of whole cell extract. 4. Chill 1X buffer on ice and add PMSF just prior to use. NOTE: Most antibodies from Cell Signaling Technology work optimally between 1 and 2 ug per IP sample. In the case where there are multiple samples with varying concentrations, … Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film. If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.If you're looking for a career that you can feel good about with a company that cares about you, the environment, and our communities, look no further than Cell Signaling Technology. Check out our current career … The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro (2,3). IHC-validated Antibodies for Cellular Senescence. Accelerate and increase confidence in your research results with IHC-validated p16 INK4A (BC42). An antibody shouldn’t be one of the variables in your experiment. Find out why customers …If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation. 抗体不应成为您实验中的变量之一。了解客户为何在抗体特异性和敏感性方面给予 cst 最高评价。 We would like to show you a description here but the site won’t allow us. For reproducible data, Cell Signaling Technology (CST) offers rigorously validated and reliable products including antibodies, reagents, and kits for biological research. We have a substantial portfolio of products to interrogate mechanisms of cellular health and disease. Whether in the fields of cancer, immunology, infectious disease ... In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca 2+ /calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3).Storage. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 31235.At Cell Signaling Technology (CST), our goal is to provide highly specific antibodies that yield strong, specific signal with minimal background. Our scientists screen a large number of antibodies and recommend only those best suited for the application. Our validation efforts include extensive protocol optimization and antibody titrationAt Cell Signaling Technology (CST), we understand that there is no single assay that can determine the validity of an antibody.Confirming that an immunoreagent is sufficiently specific and sensitive depends on the application and protocol being used, the type and quality of sample being analyzed, and the inherent biophysical properties of the antibody itself.PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197 and 1217 is correlated with PLCγ2 activity (9,10). Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509. Smrcka, A.V. et al. (1991) Science 251, 804-7.Poster: Signaling Pathways | Cell Signaling Technology. This poster provides a comprehensive overview of the major signaling pathways involved in cell growth, survival, differentiation, and stress responses. Download the PDF to explore the key molecules and interactions that regulate these pathways and their implications for human health and …Cell signaling is a fundamental property of all cellular life in prokaryotes and eukaryotes . Typically, the signaling process involves three components: the signal, the receptor, and the effector. [citation needed] In biology, signals are mostly chemical in nature, but can also be physical cues such as pressure, voltage, temperature, or light. Our Approach/Process. As a privately held company founded and led by active research scientists, we understand your needs as a researcher. Like you, we only want to use products that are specific and deliver reproducible results. We validate every one of our thousands of antibodies and research products in-house -- and against multiple ... If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.Monoclonal Antibody for studying Flag-Tag. Cited in 478 publications. Validated for WB, IP, IHC, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, DYKDDDDK Tag (9A3) Mouse Monoclonal Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) (CST #8146) is ready to ship.Cell Signaling Technology has an overall rating of 4.5 out of 5, based on over 132 reviews left anonymously by employees. 96% of employees would recommend working at Cell Signaling Technology to a friend and 96% have a positive outlook for the business. This rating has improved by 2% over the last 12 months.Monoclonal Antibody for studying Flag-Tag. Cited in 478 publications. Validated for WB, IP, IHC, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, DYKDDDDK Tag (9A3) Mouse Monoclonal Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) (CST #8146) is ready to ship.Western blot analysis of extracts from CAD cells and neonatal mouse brain using Synapsin-1 (D12G5) XP ® Rabbit mAb. Simple Western™ analysis of lysates (0.1 mg/mL) from CAD cells using Synapsin-1 (D12G5) XP Rabbit mAb #5297. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. Checking the Cell Signaling Technology ® (CST ®) website, you are in luck! There are mouse reactive flow-validated antibody conjugates available against each target: CD4 and TCRβ . While you know that each protein is found at the plasma membrane, you need to check whether the antibodies recognize extracellular or intracellular portions of each. Contact Technical Support. Hours: 8:00 AM - 6:00 PM (EST) Phone: 877-678-8324. Email: [email protected]. Product Description. The Senescence β-Galactosidase Staining Kit is designed to conveniently provide reagents needed to detect β-galactosidase activity at pH 6, a known characteristic of senescent cells. Papers have published using this kit in both cells and frozen tissue. The kit includes all reagents necessary for this assay.Immunoprecipitation of ATF-6 from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is ATF-6 (D4Z8V) Rabbit mAb. Western blot analysis was performed using ATF-6 (D4Z8V) Rabbit mAb. Western blot analysis of extracts from 293T cells, untreated (-) or treated with tunicamycin (1 mM ...At Cell Signaling Technology (CST), we understand that there is no single assay that can determine the validity of an antibody.Confirming that an immunoreagent is sufficiently specific and sensitive depends on the application and protocol being used, the type and quality of sample being analyzed, and the inherent biophysical properties of the antibody itself. Support Information. Please select the team or topic you would like to reach out to for support: 1X Animal-Free Blocking Solution: to 4 mL of dH O add 1 ml of Animal-Free Blocking Solution (5X) ( #15019 ). Incubate sections in three washes of xylene for 5 minutes each. Incubate sections in two washes of 100% ethanol for 10 minutes each. Incubate sections in two washes of 95% ethanol for 10 minutes each.The innate immune system mainly consists of phagocytic cells, such as neutrophils, macrophages, natural killer cells, basophils, and others that target dangerous invading organisms. The cells of the adaptive immune system are T cells and B cells. Natural killer (NK) T cells have features of both innate and adaptive immune cells.Monoclonal Antibody for studying Flag-Tag. Cited in 478 publications. Validated for WB, IP, IHC, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, DYKDDDDK Tag (9A3) Mouse Monoclonal Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) (CST #8146) is ready to ship.Cell Signaling Technology (CST) | 21,261 followers on LinkedIn. CST is a private, family-owned company, founded by scientists and dedicated to providing high-quality research tools. | Founded by ...Specificity and sensitivity of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody. The antibody reacts specifically with as little as 0.25 ng of phosphorylated p42 MAP kinase and does not cross-react with up to 4 µg of nonphosphorylated p42 MAP kinase. Western blot analysis of whole-cell extracts from unstarved wild-type mouse embryonic ...Science & Development. As a company of scientists for scientists, we are passionate about the discovery and development of innovative therapies for human disease. Help advance …CD22 (also known as siglec-2) is a member of the sialic acid-binding immunoglobulin-type lectin (Siglec) family of immunomodulatory receptors. CD22 can bind to its ligand α 2,6-linked sialic acid on different cells (trans interaction) as well as on the same cells (cis interaction). CD22 is predominantly expressed on B cells and functions as an ...The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli, including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is ...Learn & Support. Use this Directory to navigate to a wealth of informative resources about molecular targets and the protocols and tools needed to study them efficiently. Under …Polyclonal Antibody for studying H3 (Ser10) phosphate. Cited in 1110 publications. Validated for WB, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Histone H3 (Ser10) Antibody (CST #9701) is ready to ship.Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows ...If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.Background. TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein and other cytokines including IL-1 (1,2). In vivo activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4).The biological functions of HSP70/HSP90 extend beyond their chaperone activity. They are essential for the maturation and inactivation of nuclear hormones and other signaling molecules (1,3). They also play a role in vesicle formation and protein trafficking (2). Nollen, E.A. and Morimoto, R.I. (2002) J. Cell Sci. 115, 2809-2816.Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min. Immunoprecipitation of STING from HL-60 cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or STING (D2P2F) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using STING (D2P2F) Rabbit mAb. Lane 1 is 10% input, lane 2 is USP10 (D7A5) Rabbit mAb #8501, and lane 3 is Rabbit (DA1E) mAb IgG XP ® Isotype Control. Western blot analysis was perfomed using USP10 (D7A5) Rabbit mAb #8501. Anti-rabbit IgG, HRP …Cell Signaling Technology (CST) is dedicated to providing the world’s highest quality, innovative research products to accelerate biological understanding. A privately …Monoclonal Antibody for studying LC3B. Cited in 138 publications. Validated for WB, IP, IHC, IF, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, LC3B (E5Q2K) Mouse Monoclonal Antibody (CST #83506) is ready to ship.Contact Technical Support. Hours: 8:00 AM - 6:00 PM (EST) Phone: 877-678-8324. Email: [email protected] goal is to understand how signaling pathways are integrated at the molecular and systems levels to control cellular responses. We have two main focuses: First, we study …Pin1 is a small (18 kDa) protein with two distinct functional domains: an amino-terminal WW domain and a carboxy-terminal PPlase domain. Pin1 interacts with several mitotic phosphoproteins, including Plk1, cdc25C and cdc27, and is thought to act as a phosphorylation-dependent PPlase for these target molecules (7-9).If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows ... Application Key: WB-Western IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry Species Cross-Reactivity Key: At Cell Signaling Technology (CST), we understand that there is no single assay that can determine the validity of an antibody.Confirming that an immunoreagent is sufficiently specific and sensitive depends on the application and protocol being used, the type and quality of sample being analyzed, and the inherent biophysical properties of the antibody itself.Lane 1 is 10% input, lane 2 is USP10 (D7A5) Rabbit mAb #8501, and lane 3 is Rabbit (DA1E) mAb IgG XP ® Isotype Control. Western blot analysis was perfomed using USP10 (D7A5) Rabbit mAb #8501. Anti-rabbit IgG, HRP …Directions for Use: Wash membrane-bound HRP (antibody conjugate) three times for 5 minutes in TBST. Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Refine Results. In the CST video library, you can watch how-to videos from the Tech Tips series to improve your technique, research webinars presented by scientists from CST and other institutions, and videos relevant for specific applications or disease research areas. Monoclonal Antibody for studying H3 (Ser10) phosphate. Cited in 372 publications. Validated for WB, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit Monoclonal Antibody (CST #3377) is …Cell Signaling Technology has an overall rating of 4.5 out of 5, based on over 132 reviews left anonymously by employees. 96% of employees would recommend working at Cell Signaling Technology to a friend and 96% have a positive outlook for the business. This rating has improved by 2% over the last 12 months.Simple Western™ analysis of lysates (0.1 mg/mL) from COS-7 cells, transfected with a construct expressing HA-tagged Stat3 using HA-Tag (6E2) Mouse mAb #2367. The virtual lane view (left) shows a single target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody.About Cell Signaling Technology Cell Signaling Technology (CST) is a different kind of life sciences company—one founded, owned, and run by active research scientists, with the highest standards of product and service quality, technological innovation, and scientific rigor. Founded in 1999 and headquartered in Danvers, …Immunoprecipitation of Bax from HepG2 cells. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is Bax (D2E11) Rabbit mAb, #5023. Western blot was performed …Cell Signaling Technology is a private, family-owned company that operates in the field of applied systems biology research, particularly focusing on cancer. The company's main offerings include the development and production of antibodies, which are validated for multiple applications by their team of Ph.D. level scientists.The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. CUT&Tag was performed with Hep G2 cells treated with Thapsigargin #12758 (300nM) for 4h and ATF-4 (D4B8) Rabbit mAb, using CUT&Tag Assay Kit #77552. Specificity / Sensitivity. Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. Cathepsin B (D1C7Y) XP ® Rabbit mAb recognizes endogenous levels of total cathepsin B protein.Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows ...Pathways and Diagrams. Quickly identify your next research target using the interactive pathways, diagrams, guides and cellular landscapes in each of these collections. These resources provide insights into relevant cellular processes, structures and related diseases to help you identify related proteins and cellular mechanisms to investigate ...Species Cross-Reactivity Key: Polyclonal Antibody for studying eNOS. Cited in 167 publications. Validated for Western Blotting. Highly specific and rigorously validated in-house, eNOS Antibody (CST #9572) is ready to ship.Application Key: WB-Western IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry Species Cross-Reactivity Key: H-Human M-Mouse R-Rat Hm-Hamster Mk-Monkey Vir-Virus Mi-Mink C-Chicken Dm-D. melanogaster X-Xenopus Z-Zebrafish B-Bovine Dg-Dog Pg-Pig Sc-S. …Ralphs italian ice, Hirts garden, Schneider auctions, Albert tate, West end obgyn, Hadwin white subaru, Black doctors near me, Terre haute regional hospital, American dealer, Sunshine studios, Lowes loganville ga, Together women rise, Mega motorsports, Awesome playground

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Pathways and Diagrams. Quickly identify your next research target using the interactive pathways, diagrams, guides and cellular landscapes in each of these collections. These resources provide insights into relevant cellular processes, structures and related diseases to help you identify related proteins and cellular mechanisms to investigate ...Specificity and sensitivity of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody. The antibody reacts specifically with as little as 0.25 ng of phosphorylated p42 MAP kinase and does not cross-react with up to 4 µg of nonphosphorylated p42 MAP kinase. Western blot analysis of whole-cell extracts from unstarved wild-type mouse embryonic ...Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63411.Keratin 19 (K19) is expressed in glandular epithelia, including the liver, gallbladder, and pancreas, as well as in adenocarcinomas of the breast, thyroid, and bile duct. Keratin 20 (K20) is expressed in gastrointestinal epithelium, urothelium, and Merkel cells in the skin, as well as in colorectal carcinomas and some urothelial carcinomas.Partnerships & Licensing. As a trusted partner, CST is focused on building symbiotic relationships to maximize the value of products, technology, and intellectual property; whether ours or our partner’s. True innovation is collaborative. We have broad interest in technology licensing, scientific and technical collaborations, and commercial ...Western blot analysis of extracts from various cell lines using GAPDH (14C10) Rabbit mAb. Simple Western™ analysis of lysates (0.01 mg/mL) from HeLa cells using GAPDH (14C10) Rabbit mAb #2118. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody.Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells (6,7).Lane 1 is 10% input, lane 2 is USP18 (D4E7) Rabbit mAb, and lane 3 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900. Western blot analysis was perfomed using USP18 (D4E7) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody. To Purchase # 4813. Cat.The LC3B (D11) XP ® Rabbit mAb confirms silencing of LC3B expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of LC3B siRNA. Show More. Simple Western™ analysis of lysates (1mg/ml) from HeLa cells treated with Chloroquine (50uM, O/N) using LC3B (D11) XP ® Rabbit mAb #3868.CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and … Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63411. Western blot analysis of extracts from various cell lines using Alix (E6P9B) Rabbit mAb. Immunoprecipitation of Alix from K-562 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control, and lane 3 is Alix (E6P9B) Rabbit mAb. Western blot was performed using Alix (E6P9B) Rabbit mAb.A complete, step-by-step walkthrough of the CUT&RUN Protocol included with the CUT&RUN Assay kit (#86652) from Cell Signaling Technology (CST). • Visit the ...Immunoprecipitation of TIM-3 from RPMI 8226 cell extracts. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is TIM-3 (D5D5R ™) XP ® Rabbit mAb, #45208. Western blot was performed using TIM-3 (D5D5R ™) XP ® Rabbit mAb.The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro (2,3).Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.About Cell Signaling Technology Cell Signaling Technology (CST) is a different kind of life sciences company—one founded, owned, and run by active research scientists, with the highest standards of product and service quality, technological innovation, and scientific rigor. Founded in 1999 and headquartered in Danvers, …We would like to show you a description here but the site won’t allow us.Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses, including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182.The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli, including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is ...In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca 2+ /calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3).Application Key: WB-Western IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry Species Cross-Reactivity Key: H-Human M-Mouse R-Rat Hm-Hamster Mk-Monkey Vir-Virus Mi-Mink C-Chicken Dm-D. melanogaster X-Xenopus Z-Zebrafish B-Bovine Dg-Dog Pg-Pig Sc-S. …Monoclonal Antibody for studying Flag-Tag. Cited in 478 publications. Validated for WB, IP, IHC, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, DYKDDDDK Tag (9A3) Mouse Monoclonal Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) (CST #8146) is ready to ship. For reproducible data, Cell Signaling Technology (CST) offers rigorously validated and reliable products including antibodies, reagents, and kits for biological research. We have a substantial portfolio of products to interrogate mechanisms of cellular health and disease. Whether in the fields of cancer, immunology, infectious disease ... The c-Myc Antibody confirms silencing of c-Myc expression, and the p42 MAPK Antibody is used to control for protein loading and siRNA specificity. Western blot analysis of extracts from HeLa, BaF3 and NBT-11 cells, using c-Myc Antibody. Simple Western™ analysis of lysates (1 mg/mL) from HeLa cells using c-Myc Antibody #9402.The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. CUT&Tag was performed with Hep G2 cells treated with Thapsigargin #12758 (300nM) for 4h and ATF-4 (D4B8) Rabbit mAb, using CUT&Tag Assay Kit #77552.Cell Signaling Technology (CST) | 21,261 followers on LinkedIn. CST is a private, family-owned company, founded by scientists and dedicated to providing high-quality research tools. | Founded by ...Monoclonal Antibody for studying LC3B. Cited in 138 publications. Validated for WB, IP, IHC, IF, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, LC3B (E5Q2K) Mouse Monoclonal Antibody (CST #83506) is ready to ship.Phospho-Akt substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery. Montminy, M. (1997) Annu Rev Biochem 66, 807-22. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, …Cell-cell signaling involves the transmission of a signal from a sending cell to a receiving cell. However, not all sending and receiving cells are next-door neighbors, nor do all cell pairs exchange signals in the same way. There are four basic categories of chemical signaling found in multicellular organisms: paracrine signaling, autocrine ...Background. TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein and other cytokines including IL-1 (1,2). In vivo activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4).Hr -Horse. -Guinea Pig. -Rabbit. -All Species Expected. Monoclonal Antibody for studying NeuN. Cited in 47 publications. Validated for WB, IP, IF. Highly specific and rigorously validated in-house, NeuN (E4M5P) Mouse Monoclonal Antibody (CST …Cell Signaling Technology is a private, family-owned company that operates in the field of applied systems biology research, particularly focusing on cancer. The company's main offerings include the development and production of antibodies, which are validated for multiple applications by their team of Ph.D. level scientists.Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. Cathepsin B (D1C7Y) XP ® Rabbit mAb recognizes endogenous levels of total cathepsin B protein. If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63411. Application Key: WB-Western IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry Species Cross-Reactivity Key: H-Human M-Mouse R-Rat Hm-Hamster Mk-Monkey Vir-Virus Mi-Mink C-Chicken Dm-D. melanogaster X-Xenopus Z-Zebrafish B-Bovine Dg-Dog Pg-Pig Sc-S. cerevisiae Ce-C. …Checking the Cell Signaling Technology ® (CST ®) website, you are in luck! There are mouse reactive flow-validated antibody conjugates available against each target: CD4 … Our Approach/Process. As a privately held company founded and led by active research scientists, we understand your needs as a researcher. Like you, we only want to use products that are specific and deliver reproducible results. We validate every one of our thousands of antibodies and research products in-house -- and against multiple ... Lyse cells by adding 1X SDS sample buffer (100 µl per well of 6-well plate or 500 µl for a 10 cm diameter plate). Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity).Monoclonal Antibody for studying ACTA2 (alpha actin, smooth muscle). Cited in 560 publications. Validated for WB, WB, IP, IHC, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, α-Smooth Muscle Actin (D4K9N) XP® Rabbit Monoclonal Antibody (CST #19245) is ready to ship.At Cell Signaling Technology (CST), our goal is to provide highly specific antibodies that yield strong, specific signal with minimal background. Our scientists screen a large number of antibodies and recommend only those best suited for the application. Our validation efforts include extensive protocol optimization and antibody titrationPathways and Diagrams. Quickly identify your next research target using the interactive pathways, diagrams, guides and cellular landscapes in each of these collections. These resources provide insights into relevant cellular processes, structures and related diseases to help you identify related proteins and cellular mechanisms to investigate ...The matrix metalloproteinases (MMPs) are a family of proteases that target many extracellular proteins including other proteases, growth factors, cell surface receptors, and adhesion molecules (1). Among the family members, MMP-2, MMP-3, MMP-7, and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic ...Cell Signaling Technology (CST) is a different kind of life sciences company—one founded, owned, and run by active research scientists, with the highest standards …Checking the Cell Signaling Technology ® (CST ®) website, you are in luck! There are mouse reactive flow-validated antibody conjugates available against each target: CD4 … CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the ... Western blot analysis of extracts from control HeLa cells (lane 1) or Caspase-8 knockout HeLa cells (lane 2) using Caspase-8 (1C12) Mouse mAb #9746 (upper), or β-actin (13E5) Rabbit mAb #4970 (lower). The absence of signal in the Caspase-8-knockout HeLa cells confirms specificity of the antibody for Caspase-8.Immunohistochemistry (IHC) staining allows for 2 broad classes of detection: 1) chromogenic and 2) fluorescent. For chromogenic detection, CST recommends using polymer-based systems that avoid the limitations of the biotin-based system while also increasing sensitivity of the assay. Try SignalStain ® Boost IHC Detection Reagent (HRP, … Rabbit IgG. Monoclonal Antibody for studying LAMP1. Cited in 477 publications. Validated for WB, IP, IHC, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, LAMP1 (D2D11) XP® Rabbit Monoclonal Antibody (CST #9091) is ready to ship. Mar 14, 2023 · A complete, step-by-step walkthrough of the CUT&RUN Protocol included with the CUT&RUN Assay kit (#86652) from Cell Signaling Technology (CST). • Visit the ... Mar 14, 2023 · A complete, step-by-step walkthrough of the CUT&RUN Protocol included with the CUT&RUN Assay kit (#86652) from Cell Signaling Technology (CST). • Visit the ... Specificity / Sensitivity. Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full-length caspase-3 or other cleaved caspases.Cyclin D is degraded through the ubiquitin proteasome pathway in the absence of mitogenic signaling. Ubiquitination of cyclin D1 is enhanced by phosphorylation at Thr286 by glycogen synthase kinase 3b (GSK-3b) (1). p27/Kip1, p57 Kip2 and p21 Waf1/Cip1 are members of the Cip/Kip family of cyclin-dependent kinase inhibitors.At Cell Signaling Technology (CST), we understand that there is no single assay that can determine the validity of an antibody.Confirming that an immunoreagent is sufficiently specific and sensitive depends on the application and protocol being used, the type and quality of sample being analyzed, and the inherent biophysical properties of the antibody itself.Western blot analysis of extracts from various cell lines using GCN5L2 (C26A10) Rabbit mAb. Confocal immunofluorescent analysis of HeLa cells using GCN5L2 (C26A10) Rabbit mAb (green). Actin filaments have been labeled with DY554 phalloidin (red). Blue pseudocolor = DRAQ5 ™ (fluorescent DNA dye). To Purchase # 3305. Cat.If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min. 抗体不应成为您实验中的变量之一。了解客户为何在抗体特异性和敏感性方面给予 cst 最高评价。 Product Description. This Cell Signaling Technology antibody is tested in-house for indirect flow cytometric analysis in human cells and is expected to react with cell surface expressed CARs of varying specificity, which contain a G4S linker within the scFv of the extracellular domain. Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.A complete, step-by-step walkthrough of the CUT&RUN Protocol included with the CUT&RUN Assay kit (#86652) from Cell Signaling Technology (CST). • Visit the ...Western blot analysis of extracts from various cell lines using GAPDH (14C10) Rabbit mAb. Simple Western™ analysis of lysates (0.01 mg/mL) from HeLa cells using GAPDH (14C10) Rabbit mAb #2118. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. Specificity / Sensitivity. Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins. Receptors are the main switches initiating downstream short-range and long-distance signaling in the cell and modulating cellular activities (Czyzewicz et al. …Confocal immunofluorescent analysis of HEK293 (positive, left) and PANC-1 (negative, right) cells, using NKX2.5 (E1Y8H) Rabbit mAb (green). Actin filaments were labeled with DyLight ™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).Immunohistochemistry (IHC) staining allows for 2 broad classes of detection: 1) chromogenic and 2) fluorescent. For chromogenic detection, CST recommends using polymer-based systems that avoid the limitations of the biotin-based system while also increasing sensitivity of the assay. Try SignalStain ® Boost IHC Detection Reagent (HRP, …. 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